Fecker L F, Rügenhagen C, Berlin J
Institut für Biochemie und Pflanzenvirologie, Biologische Bundesanstalt für Land- und Forstwirtschaft, Braunschweig, Germany.
Plant Mol Biol. 1993 Oct;23(1):11-21. doi: 10.1007/BF00021415.
Several hairy root cultures of Nicotiana tabacum varieties, carrying two direct repeats of a bacterial lysine decarboxylase (ldc) gene controlled by the cauliflower mosaic virus (CaMV) 35S promoter expressed LDC activity up to 1 pkat/mg protein. Such activity was, for example, sufficient to increase cadaverine levels of the best line SR3/1-K1,2 from ca. 50 micrograms (control cultures) to about 700 micrograms/g dry mass. Some of the overproduced cadaverine of this line was used for the formation of anabasine, as shown by a 3-fold increase of this alkaloid. In transgenic lines with lower LDC activity the changes of cadaverine and anabasine levels were correspondingly lower and sometimes hardly distinguishable from controls. Feeding of lysine to root cultures, even to those with low LDC activity, greatly enhanced cadaverine and anabasine levels, while the amino acid had no or very little effect on controls and LDC-negative lines.
几种烟草品种的毛状根培养物，携带由花椰菜花叶病毒（CaMV）35S启动子控制的细菌赖氨酸脱羧酶（ldc）基因的两个直接重复序列，其LDC活性高达1 pkat/mg蛋白质。例如，这样的活性足以使最佳品系SR3/1-K1,2的尸胺水平从约50微克（对照培养物）增加到约700微克/克干重。该品系一些过量产生的尸胺用于形成新烟草碱，这种生物碱增加了3倍就证明了这一点。在LDC活性较低 的转基因品系中，尸胺和新烟草碱水平的变化相应较低，有时与对照几乎没有区别。向根培养物中添加赖氨酸，即使是添加到LDC活性较低的培养物中，也能大大提高尸胺和新烟草碱水平，而这种氨基酸对对照和LDC阴性品系没有影响或影响很小。
