Ainsworth C, Tarvis M, Clark J
Department of Biological Sciences, Wye College, University of London, Kent, UK.
Plant Mol Biol. 1993 Oct;23(1):23-33. doi: 10.1007/BF00021416.
A full-length cDNA clone from hexaploid bread wheat, encoding the small subunit of ADP-glucose pyrophosphorylase, has been isolated from an endosperm cDNA library. The cDNA insert has an open reading frame which encodes a protein of 473 amino acids (52.1 kDa). The presence of a chloroplast/amyloplast transit peptide of 22 amino acids is proposed. The deduced amino acid sequence exhibits a high degree of homology with the small subunit ADP-glucose pyrophosphorylase proteins from rice (with 90% of identical amino acids) and potato (with 86% of identical amino acids) and contains conserved sequence elements which are thought to represent the substrate binding and allosteric activator sites. The genes are organised as single-copy loci on chromosomes 7A, 7B and 7D in the wheat genome and are highly expressed during grain development. Homologous transcripts are expressed in leaves and roots.
从六倍体面包小麦的胚乳cDNA文库中分离出了一个全长cDNA克隆，该克隆编码ADP-葡萄糖焦磷酸化酶的小亚基。cDNA插入片段有一个开放阅读框，编码一个由473个氨基酸组成的蛋白质（52.1 kDa）。推测存在一个由22个氨基酸组成的叶绿体/造粉体转运肽。推导的氨基酸序列与来自水稻（90%氨基酸相同）和马铃薯（86%氨基酸相同）的ADP-葡萄糖焦磷酸化酶小亚基蛋白具有高度同源性，并包含被认为代表底物结合和变构激活位点的保守序列元件。这些基因在小麦基因组中作为单拷贝基因座排列在7A、7B和7D染色体上，在籽粒发育过程中高度表达。同源转录本在叶片和根中表达。
