Lin E, Burns D J, Gardner R C
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Plant Mol Biol. 1993 Nov;23(3):489-99. doi: 10.1007/BF00019297.
We have examined the expression of actinidin, a cysteine protease found in kiwifruit, over the course of fruit development. Protease activity was first seen in fruit that had reached about half their final weight, and rose to high levels at harvest. The 5'-flanking region (nucleotides -1301 to +58) of a kiwifruit actinidin gene was fused to the beta-glucuronidase (GUS)-coding region, and the chimaeric gene was introduced into transgenic petunia plants. Induction of the GUS gene was observed during the later stages of seed pod development, closely resembling the pattern of actinidin induction in fruit tissues of kiwifruit. Some GUS expression was also detected in the vascular system of the receptacle, leaves, stems and roots. A shorter promoter fragment consisting of nucleotides -115 to +58 conferred similar spatial and temporal regulation in some of the transgenic plants.
我们研究了猕猴桃中发现的半胱氨酸蛋白酶——肌动蛋白水解酶在果实发育过程中的表达情况。蛋白酶活性首先在达到最终重量约一半的果实中出现，并在收获时升至高水平。将猕猴桃肌动蛋白水解酶基因的5'侧翼区域（核苷酸-1301至+58）与β-葡萄糖醛酸酶（GUS）编码区域融合，并将嵌合基因导入转基因矮牵牛植株中。在种子荚发育后期观察到GUS基因的诱导，这与猕猴桃果实组织中肌动蛋白水解酶的诱导模式非常相似。在花托、叶片、茎和根的维管系统中也检测到了一些GUS表达。由核苷酸-115至+58组成的较短启动子片段在一些转基因植物中赋予了类似的时空调控。
