Van Haaren M J, Houck C M
Calgene Inc., Davis, CA 95616.
Plant Mol Biol. 1993 Feb;21(4):625-40. doi: 10.1007/BF00014546.
The 5' region of the fruit-specific tomato gene, 2A11, contains both positive and negative regulatory elements. We divided the 5' promoter region of the 2A11 gene into small fragments, ranging in size from 211 to 634 bp and used these short DNA fragments in in vitro protein-binding studies. These studies revealed the presence of at least four fruit-specific and one leaf- and fruit-active protein-binding domains. These promoter fragments, as well as other overlapping fragments, were tested for their ability to enhance expression from a truncated heterologous promoter in transgenic plants. This analysis showed the presence of four fruit-specific and three general or leaf-active positive regulatory elements. Comparison of the results obtained with these two approaches allowed us to draw a functional map of the 2A11 promoter.
果实特异性番茄基因2A11的5'区域包含正向和负向调控元件。我们将2A11基因的5'启动子区域分成大小从211到634 bp不等的小片段，并在体外蛋白质结合研究中使用这些短DNA片段。这些研究揭示了至少四个果实特异性以及一个叶和果实活性的蛋白质结合结构域的存在。这些启动子片段以及其他重叠片段，在转基因植物中针对其增强截短的异源启动子表达的能力进行了测试。该分析表明存在四个果实特异性以及三个一般或叶活性的正向调控元件。通过比较这两种方法获得的结果，我们绘制了2A11启动子的功能图谱。
