Higgins M L
J Bacteriol. 1976 Sep;127(3):1337-45. doi: 10.1128/jb.127.3.1337-1345.1976.
A new ultrastructural technique has been developed to study the geometry of cell wall assembly in Streptococcus faecalis, which is believed to occur between pairs of raised bands located on the organism's surface. Three-dimensional reconstructions of these new regions of envelope growth are produced from the mathematical rotation (around a central axis) of various measurements taken from central, longitudinal thin sections of cells. These reconstructions can be used to calculate the surface area and volume of the septal and peripheral walls that were supposedly present in any given cell before sectioning. In an accompanying paper, it is shown how such surface and volume estimations, coupled with other measurements of length, thickness, and curvature, can be used to characterize a cycle of envelope growth in this organism. The validity of the assumptions used to reconstruct cells by rotation and the possible sources of error in using this technique are discussed.
已开发出一种新的超微结构技术来研究粪肠球菌细胞壁组装的几何学，据信这种组装发生在该生物体表面的成对隆起带之间。通过对细胞中央纵向薄片进行各种测量，并围绕中心轴进行数学旋转，从而生成这些新的包膜生长区域的三维重建图。这些重建图可用于计算在切片前任何给定细胞中假定存在的隔膜壁和外周壁的表面积和体积。在一篇配套论文中，展示了如何将这种表面积和体积估计值与长度、厚度和曲率的其他测量值相结合，以表征该生物体包膜生长的一个周期。文中还讨论了通过旋转重建细胞所使用假设的有效性以及使用该技术可能存在的误差来源。
