用于鉴定抗水疱性口炎（印第安纳3型）病毒抗体的病毒中和试验与酶联免疫吸附测定的比较

Comparison of virus neutralisation and enzyme-linked immunosorbent assay for the identification of antibodies against vesicular stomatitis (Indiana 3) virus.

作者信息

Allende R, Germano P M

机构信息

Pan American Foot-and-Mouth Disease Center (PANAFTOSA/HPV/PAHO), Rio de Janeiro, Brazil.

出版信息

Rev Sci Tech. 1993 Sep;12(3):849-55. doi: 10.20506/rst.12.3.717.

DOI:10.20506/rst.12.3.717

PMID:8219334

Abstract

Bovine, equine and swine sera from areas free from vesicular stomatitis virus (VSV) Indiana 3 (IND3)--namely Argentina, Chile, Italy and Uruguay--and endemic areas (in Brazil) were examined for anti-VSV IND3 virus antibodies in order to compare results obtained using the virus neutralisation (VN) test and liquid-phase blocking enzyme-linked immunosorbent assay (ELISA). Statistical analysis of the data showed close agreement between the two techniques (K = 0.92). The ELISA gave a higher specificity, positive predictive value and efficiency than the VN test. The ELISA offers the additional advantage of using a non-infectious antigen and can therefore be used for antibody quantification in diagnostic laboratories in VSV-free areas to support epidemiological surveillance programmes.

摘要

对来自无水泡性口炎病毒（VSV）印第安纳3型（IND3）地区（即阿根廷、智利、意大利和乌拉圭）以及（巴西的）流行地区的牛、马和猪血清进行检测，以寻找抗VSV IND3病毒抗体，以便比较使用病毒中和（VN）试验和液相阻断酶联免疫吸附测定（ELISA）所获得的结果。数据的统计分析表明，这两种技术之间具有高度一致性（K = 0.92）。ELISA比VN试验具有更高的特异性、阳性预测值和效率。ELISA还具有使用非感染性抗原的额外优势，因此可用于无VSV地区诊断实验室的抗体定量，以支持流行病学监测计划。