National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 430-824, Republic of Korea.
J Virol Methods. 2010 Mar;164(1-2):96-100. doi: 10.1016/j.jviromet.2009.12.005. Epub 2009 Dec 16.
A recombinant glycoprotein (R-GP) of vesicular stomatitis New Jersey virus (VSV-NJ) was expressed in insect cells by a baculovirus system. Its utility as a diagnostic antigen in a blocking ELISA was investigated as an alternative to the current native GP extracted from VSV-NJ. With the cut-off value of 73% inhibition, the R-GP ELISA exhibited 99.1% specificity for naive sera from cattle and horses. It did not cross-react with VSV-Indiana (VSV-IN) positive sera and differentiated from foot-and-mouth disease and swine vesicular disease. Taken together, this is the first report that the R-GP has a potential to be used as a diagnostic antigen in place of the native GP for the detection of antibodies to VSV-NJ in cattle and horses.
水疱性口炎病毒新泽西型（VSV-NJ）的重组糖蛋白（R-GP）通过杆状病毒系统在昆虫细胞中表达。研究了其作为阻断 ELISA 中的诊断抗原的用途，作为从 VSV-NJ 提取的天然 GP 的替代物。在 73%抑制的截断值下，R-GP ELISA 对牛和马的原始血清的特异性为 99.1%。它与 VSV-Indiana（VSV-IN）阳性血清不发生交叉反应，并且与口蹄疫和猪水疱病相区别。总的来说，这是首次报道 R-GP 有可能替代天然 GP 作为诊断抗原，用于检测牛和马对 VSV-NJ 的抗体。
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