Taylor R N, Fulford K M
J Clin Microbiol. 1976 Jul;4(1):32-9. doi: 10.1128/jcm.4.1.32-39.1976.
The changes in the types and numbers of tests used by participants in the Center for Disease Control Proficiency Testing Program for the detection of hepatitis B surface antigen from the beginning of the program in 1971 until October 1975 are analyzed; the implications of these changes are discussed. Changes in the use of agar gel diffusion, rheophoresis, counterelectrophoresis, complement fixation, reverse passive latex agglutination, radioimmunoassay, and reverse passive hemagglutination tests are reviewed. The performance of the participants for 1975 is reported, and factors related to performance (type or combinations of tests used, procedures used to confirm specificity, etc.) are discussed. The increase in the use of third-generation tests in place of tests with lower sensitivity and the decrease in the use of multiple tests undoubtedly represent increased efficiency and effectiveness.
分析了疾病控制中心乙肝表面抗原检测能力验证计划参与者从1971年计划开始至1975年10月期间所使用检测方法的类型和数量变化；讨论了这些变化的影响。回顾了琼脂凝胶扩散、电泳、对流电泳、补体结合、反向被动乳胶凝集、放射免疫测定和反向被动血凝试验使用情况的变化。报告了1975年参与者的表现，并讨论了与表现相关的因素（所使用检测方法的类型或组合、用于确认特异性的程序等）。使用第三代检测方法取代灵敏度较低的检测方法的增加以及多项检测使用的减少无疑代表了效率和效力的提高。
