Nakashima K, Kawaguchi S, Akiyama S, Schulman S G
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Biomed Chromatogr. 1993 Jul-Aug;7(4):217-9. doi: 10.1002/bmc.1130070409.
A high performance liquid chromatographic method with chemiluminescence detection for the determination of penicillin G and ampicillin is reported. The method is based on the enhancement of the luminol chemiluminescence with beta-lactam antibiotics. The linear relationship was obtained between the peak height and the concentration of penicillin G or ampicillin up to 15 nmol per 20 microL injection. Detection limits were 1 nmol for penicillin G and 0.5 nmol for ampicillin with a signal-to-noise ratio of 2. Relative standard deviations for five replicate measurements of 4 nmol/injection each of penicillin G and ampicillin were 2.1 and 2.3%, respectively.
报道了一种采用化学发光检测的高效液相色谱法测定青霉素G和氨苄青霉素。该方法基于β-内酰胺抗生素增强鲁米诺化学发光。进样20 μL时，青霉素G或氨苄青霉素的峰高与浓度在高达15 nmol范围内呈线性关系。青霉素G的检测限为1 nmol，氨苄青霉素的检测限为0.5 nmol，信噪比为2。青霉素G和氨苄青霉素每次进样4 nmol，进行五次重复测量的相对标准偏差分别为2.1%和2.3%。
