采用流动注射分析和高效液相色谱-化学发光检测法测定儿茶酚胺。

Determination of catecholamines by flow-injection analysis and high-performance liquid chromatography with chemiluminescence detection.

作者信息

Nalewajko Edyta, Wiszowata Aneta, Kojło Anatol

机构信息

Institute of Chemistry, University of Białystok, ul. Hurtowa 1, 15-399 Białystok, Poland.

出版信息

J Pharm Biomed Anal. 2007 Apr 11;43(5):1673-81. doi: 10.1016/j.jpba.2006.12.021. Epub 2007 Jan 5.

DOI:10.1016/j.jpba.2006.12.021

PMID:17289328

Abstract

A chemiluminescence (CL) detection of catecholamines [norepinephrine (NE), epinephrine (E), dopamine (DA) and L-dopa (LD)] is described for the flow-injection (FI) and high-performance liquid chromatographic (HPLC) determination of these compounds. The detection method is based on the inhibition effect of catecholamines (CAs) on the CL reaction of luminol with iodine in the alkaline medium. The proposed FI method allows the determination of CAs in pharmaceutical preparations for the purpose of drug quality control. The calibration curves show good linearity in the concentration range of: 1.1-20.0 microg l(-1) (NE), 0.5-5.0 microg l(-1) (E), 0.6-9.0 microg l(-1) (DA) and 0.6-10.0 microg l(-1) (LD). The limits of detection (defined as a signal-to-noise ratio of 3) are: 0.34 microg l(-1) (NE), 0.15 microg l(-1) (E) and 0.18 microg l(-1) (DA, LD). The HPLC procedure was successfully applied for the determination of catecholamines (NE, E, DA) in human urine after solid-phase extraction (SPE). In a simple run time CAs can be determined in 20 min. The chromatographic linear ranges are: 5.0-72.0 microg l(-1) (NE), 5.0-48.0 microg l(-1) (E) and 5.0-96.0 microg l(-1) (DA). The limits of detection for three urinary CAs are: 0.71 microg l(-1) (NE), 0.26 microg l(-1) (E) and 0.73 microg l(-1) (DA).

摘要

描述了一种用于流动注射（FI）和高效液相色谱（HPLC）测定儿茶酚胺类物质[去甲肾上腺素（NE）、肾上腺素（E）、多巴胺（DA）和左旋多巴（LD）]的化学发光（CL）检测方法。该检测方法基于儿茶酚胺类物质（CAs）在碱性介质中对鲁米诺与碘的CL反应的抑制作用。所提出的FI方法可用于药物制剂中CAs的测定，以进行药物质量控制。校准曲线在以下浓度范围内显示出良好的线性：1.1 - 20.0 μg l⁻¹（NE）、0.5 - 5.0 μg l⁻¹（E）、0.6 - 9.0 μg l⁻¹（DA）和0.6 - 10.0 μg l⁻¹（LD）。检测限（定义为信噪比为3）为：0.34 μg l⁻¹（NE）、0.15 μg l⁻¹（E）和0.18 μg l⁻¹（DA、LD）。HPLC方法成功应用于固相萃取（SPE）后人尿中儿茶酚胺类物质（NE、E、DA）的测定。在简单的运行时间内，20分钟即可测定CAs。色谱线性范围为：5.0 - 72.0 μg l⁻¹（NE）、5.0 - 48.0 μg l⁻¹（E）和5.0 - 96.0 μg l⁻¹（DA）。三种尿中CAs的检测限为：0.71 μg l⁻¹（NE）、0.26 μg l⁻¹（E）和0.73 μg l⁻¹（DA）。