Fukuda T, Yun A, Imai K
Tsumura & Co., Ibaraki, Japan.
Biomed Chromatogr. 1993 Jul-Aug;7(4):229-30. doi: 10.1002/bmc.1130070412.
A sensitive and selective reversed phase high performance liquid chromatographic method was introduced for the determination of salmon calcitonin (sCT) in solutions containing bovine serum albumin or gelatin. The method was based on the sensitive fluorescence detection of sCT by postcolumn derivatization with o-phthal(di)aldehyde. The sample was loaded onto a reversed phase column, purified by column switching and separated with linear gradient elution using the ion pair technique. A linear relationship was obtained between the peak height and the amount injected in the concentration range 1-40 ng. The detection limit was 10 ng/mL (S/N = 3) at a sample injection volume of 100 microL.
介绍了一种灵敏且选择性好的反相高效液相色谱法，用于测定含有牛血清白蛋白或明胶的溶液中的鲑鱼降钙素（sCT）。该方法基于邻苯二甲醛柱后衍生化对sCT进行灵敏的荧光检测。样品加载到反相柱上，通过柱切换进行纯化，并使用离子对技术进行线性梯度洗脱分离。在1 - 40 ng的浓度范围内，峰高与进样量之间呈线性关系。在进样体积为100微升时，检测限为10 ng/mL（信噪比 = 3）。
