Fukuda T, Ishikawa K, Imai K
Tsumura & Co., Ibaraki, Japan.
Biomed Chromatogr. 1995 Jan-Feb;9(1):52-5. doi: 10.1002/bmc.1130090111.
A high sensitive analytical method for salmon calcitonin (sCT) was established by means of derivatization with a fluorescence labelling agent, DBD-F, followed by HPLC separation and fluorescence detection at 558 nm with excitation at 430 nm. Under optimized conditions (DBD-F, 0.1 M borate buffer (pH 8.5):acetonitrile (70:30, v/v)), three molecules of DBD-F reacted with one sCT molecule in the presence of 1 mM sodium dodecyl sulphate (SDS) at 50 degrees C for 3 h. The detection limit for the sCT derivative was 20 fmol. A linear relationship was obtained between the amount of sCT on column and the peak heights in the range of 35-8000 fmol.
通过用荧光标记剂DBD-F进行衍生化，随后进行HPLC分离，并在430nm激发波长下于558nm处进行荧光检测，建立了一种用于鲑鱼降钙素（sCT）的高灵敏度分析方法。在优化条件下（DBD-F、0.1M硼酸盐缓冲液（pH 8.5）：乙腈（70:30，v/v）），在1mM十二烷基硫酸钠（SDS）存在下，于50℃反应3小时，三分子DBD-F与一分子sCT反应。sCT衍生物的检测限为20fmol。在35 - 8000fmol范围内，柱上sCT的量与峰高之间呈线性关系。
