Kapuściński J, Kuroszczyk J, Liniecki J, Bieńkiewicz M, Zieliński K, Tuszyner K
Department of Nuclear Medicine, University School of Medicine, Lodz, Poland.
Pol J Occup Med Environ Health. 1993;6(2):169-83.
Liver damage was induced in rabbits by graded doses of carbon tetrachloride. The damage was assessed by means of quantitative morphometry using necrosis and steatosis of the parenchyma as end points. Biological response was measured utilizing a series of typical biochemical indices of liver damage as well as plasma clearance by the liver 99mTc-mebrofenin (an IDA derivative) and quantified indices of uptake and organ transfer of the compound. No correlation was found between the CCl4 dose and its effect (i.e. degree of necrosis and steatosis of liver). However, a very strong negative correlation was found between the degree of necrosis in individual animals and value of the clearance. The extent of the damage was positively correlated with liver transfer rate of 99mTc-MBF. Plasma activity of ALAT, TG, GGTP and AspAT were higher in the poisoned animals, however, only increments of activity of GGTP, ALAT and LDH showed statistically significant correlation with the individually assessed damage (necrosis).
通过给兔子注射不同剂量的四氯化碳诱导肝损伤。以实质细胞的坏死和脂肪变性为终点，采用定量形态学方法评估损伤程度。利用一系列典型的肝损伤生化指标以及肝脏对99mTc-美布芬宁（一种IDA衍生物）的血浆清除率和该化合物的摄取及器官转运量化指标来测量生物学反应。未发现四氯化碳剂量与其效应（即肝脏坏死和脂肪变性程度）之间存在相关性。然而，在个体动物中，坏死程度与清除率值之间存在非常强的负相关性。损伤程度与99mTc-MBF的肝脏转运率呈正相关。中毒动物的ALAT、TG、GGTP和AspAT血浆活性较高，然而，只有GGTP、ALAT和LDH活性的增加与个体评估的损伤（坏死）显示出统计学上的显著相关性。
