Nicolai U, Eckardt C
Augenklinik Frankfurt-Höchst, Frankfurt am Main, Germany.
Ger J Ophthalmol. 1993 Aug;2(4-5):195-201.
Specimens obtained from eyes with various ocular diseases were examined immunohistochemically using a panel of monoclonal antibodies (Ki-M1P, K1-M4, CD68, anti-lysozyme, and anti-cytokeratins) to establish the occurrence of macrophages in the retina and periretinal tissues and to determine whether they can be distinguished from migrated cells of the retinal pigment epithelium (RPE). Eyes with proliferative diseases and intraocular melanomas were found to contain more macrophages in the retina than those with uveitis and glaucoma. The epiretinal membranes and subretinal space in eyes of the former group often exhibited additional clustered macrophages and migrated RPE cells. In all specimens studied, RPE cells reacted with antibodies KL-1, CK7, CK18, or CK19 but not with the CD68, Ki-M1P, or lysozyme antibodies. Control eyes without any known disease also contained rare macrophages in the retina. In conclusion, macrophages are regular and normal constituents of the retina that can be clearly distinguished immunohistochemically from migrated RPE cells. Their frequency varies depending on the type of ocular disease involved.
使用一组单克隆抗体（Ki-M1P、K1-M4、CD68、抗溶菌酶和抗细胞角蛋白）对取自患有各种眼部疾病眼睛的标本进行免疫组织化学检查，以确定视网膜和视网膜周围组织中巨噬细胞的存在情况，并确定它们是否可以与视网膜色素上皮（RPE）的迁移细胞区分开来。发现患有增殖性疾病和眼内黑色素瘤的眼睛视网膜中的巨噬细胞比患有葡萄膜炎和青光眼的眼睛更多。前一组眼睛的视网膜前膜和视网膜下间隙通常表现出额外的聚集巨噬细胞和迁移的RPE细胞。在所有研究的标本中，RPE细胞与抗体KL-1、CK7、CK18或CK19发生反应，但不与CD68、Ki-M1P或溶菌酶抗体发生反应。没有任何已知疾病的对照眼视网膜中也含有罕见的巨噬细胞。总之，巨噬细胞是视网膜的正常组成成分，可以通过免疫组织化学方法与迁移的RPE细胞清楚地区分开来。它们的频率因所涉及的眼部疾病类型而异。
