Lee K A, Goepel J R, Winfield D A, Hancock B W, Goyns M H
Department of Clinical Oncology, Royal Hallamshire Hospital, Sheffield, UK.
Leuk Lymphoma. 1993 Sep;11(1-2):91-8. doi: 10.3109/10428199309054734.
Rearrangements of the BCL-2 gene are thought to be the most frequent genetic changes that occur in non-Hodgkin's lymphomas (NHL), and are particularly associated with follicular low grade disease. Wide variations in the frequency of these rearrangements have, however, been reported in studies of NHL series from different parts of the world. We were therefore interested to determine the frequency of BCL-2 gene rearrangements in the different grades of NHL from a U.K. series. We have done this by using a combination of Southern blot hybridization and polymerase chain reaction (PCR) analysis. The frequencies of rearrangements in our series were 9/20 (45%) in low grade follicular NHL, 1/8 (12.5%) in low grade lymphocytic and 5/19 (26%) in high grade NHL. However, estimation of the high grade value was complicated by the fact that a number of the high grade samples in our series were from patients who had transformed from low grade follicular disease. If the patients were ranked on the basis of whether they had a history of low grade follicular disease then the frequency of BCL-2 rearrangement remained the same 13/29 (45%), but was only 1/10 (10%) in high grade NHL with no history of follicular disease. The former figure was intermediate between those reported for the USA and Japan series, but the latter high grade figure was the lowest reported from any series. The significance of BCL-2 rearrangements in the evolution of both low and high grade NHL is discussed.
BCL-2基因重排被认为是非霍奇金淋巴瘤（NHL）中最常见的基因变化，尤其与滤泡性低级别疾病相关。然而，在世界各地不同NHL系列研究中，这些重排的频率报告存在很大差异。因此，我们有兴趣确定来自英国系列不同级别的NHL中BCL-2基因重排的频率。我们通过结合Southern印迹杂交和聚合酶链反应（PCR）分析来完成此项工作。在我们的系列中，低级别滤泡性NHL的重排频率为9/20（45%），低级别淋巴细胞性为1/8（12.5%），高级别NHL为5/19（26%）。然而，我们系列中的一些高级别样本来自于从低级别滤泡性疾病转化而来的患者，这一事实使高级别数据的估算变得复杂。如果根据患者是否有低级别滤泡性疾病史进行排序，那么BCL-2重排的频率保持不变，为13/29（45%），但在无滤泡性疾病史的高级别NHL中仅为1/10（10%）。前一个数字介于美国和日本系列报告的数据之间，但后一个高级别数字是所有系列中报告的最低值。本文讨论了BCL-2重排在低级别和高级别NHL演变中的意义。
