Métézeau P
Service INSERM-Pasteur de Cytométrie analytique et préparative, CNRS, URA 1129, Paris, France.
Biol Cell. 1993;78(1-2):129-34. doi: 10.1016/0248-4900(93)90123-v.
Flow cytometry (FMC) is an analytical and preparative technique whereas image analysis is only applied to cell analysis. Recently, image analysis has been adapted as a preparative method using a new technique: image cytometry for analysis and sorting (ICAS). FCM and ICAS are complementary. Flow cytometry allows rapid, quantitative and precise study of fluorescence and light scattering in a large number of cells in suspension, while ICAS analyses fewer cells (adherent cells or tissue) on the basis of fluorescence, morphology and size. ICAS can use these criteria to destroy unwanted cells and hence sort selected cells. ICAS can also be used for confocal microscopy and laser surgery.
流式细胞术（FMC）是一种分析和制备技术，而图像分析仅应用于细胞分析。最近，图像分析已通过一种新技术被用作一种制备方法：用于分析和分选的图像细胞术（ICAS）。流式细胞术和图像细胞术是互补的。流式细胞术能够对大量悬浮细胞中的荧光和光散射进行快速、定量和精确的研究，而图像细胞术则基于荧光、形态和大小分析较少数量的细胞（贴壁细胞或组织）。图像细胞术可以利用这些标准来破坏不需要的细胞，从而分选选定的细胞。图像细胞术还可用于共聚焦显微镜检查和激光手术。
