Section of Cardiology, Department of Internal Medicine, Yale Stem Cell Center, Yale School of Medicine, Yale University, New Haven, Conn. 06519, USA.
Cells Tissues Organs. 2012;195(1-2):26-40. doi: 10.1159/000331390. Epub 2011 Oct 12.
Research in cell biology and the development of translational technologies are driven by competition, public expectations, and regulatory oversight, putting these fields at a critical juncture. Success in these fields is quickly becoming dependent on the ability of researchers to identify and isolate specific cell populations from heterogeneous mixtures accurately and efficiently. Many methods for cell purification have been developed, and each has advantages and disadvantages that must be considered in light of the intended application. Current cell separation strategies make use of surface proteins, genetic expression, and physics to isolate specific cells by phenotypic traits. Cell purification is also dependent on the cellular reagents available for use and the intended application, as these factors may preclude certain mechanisms used in the processes of labeling and sorting cells.
细胞生物学研究和转化技术的发展受到竞争、公众期望和监管监督的推动，使这些领域处于关键的转折点。这些领域的成功与否越来越取决于研究人员识别和分离特定细胞群体的能力，需要从异质混合物中快速、准确、高效地分离。已经开发出许多细胞纯化方法，每种方法都有其优点和缺点，必须根据预期的应用来考虑。目前的细胞分离策略利用表面蛋白、基因表达和物理特性来根据表型特征分离特定的细胞。细胞纯化还取决于可用于细胞的试剂和预期的应用，因为这些因素可能会排除细胞标记和分选过程中使用的某些机制。
