Sosa A, Celis H
Departamento de Bioenergética, Universidad Nacional Autónoma de México, México, D.F.
Biochem Mol Biol Int. 1993 Aug;30(6):1135-41.
The surface charge of the membrane of chromatophores of Rhodospirillum rubrum was modified by two methods: fusion of liposomes with the membrane of the chromatophore by changing the pH and by incubating chromatophores in the presence of cationic or anionic detergents. The hydrolytic activity of membrane-bound pyrophosphatase, on surface charge modified chromatophores, did not change the Km of the enzyme for its substrate (Mg-PPi2-) nor the activation effect of free Mg2+ on the hydrolytic activity. This membrane enzyme is not regulated by surface charge.
通过改变pH值使脂质体与载色体膜融合，以及在阳离子或阴离子去污剂存在的情况下孵育载色体。膜结合焦磷酸酶在表面电荷修饰的载色体上的水解活性，既没有改变该酶对其底物（Mg-PPi2-）的Km值，也没有改变游离Mg2+对水解活性的激活作用。这种膜酶不受表面电荷的调节。
