Marty I, Brugidou C, Chartier Y, Meyer Y
Laboratoire de Biologie Moléculaire et Physiologie Végétale, Unité associée au CNRS 565, Université de Perpignan, France.
Plant J. 1993 Aug;4(2):265-78. doi: 10.1046/j.1365-313x.1993.04020265.x.
Eight cDNAs whose genes are more strongly expressed in suspension cells in growth phase than in stationary phase and at a low level in mature leaves have been isolated. The corresponding mRNAs are abundantly accumulated in young plant organs and in germinating seeds but are almost undetectable in mature plant tissues and dry seeds. Six of these cDNAs were characterized by comparison of nucleotide and protein sequences to the EMBL and SWISSPROT databanks. These eight growth-related genes are expressed in protoplasts isolated from Nicotiana tabacum mesophyll cells shortly after preparation (4 h). Two of them are expressed in freshly isolated protoplasts (early genes), while the other six are detected after 4 h of culture (late genes). Seven are more abundantly expressed in protoplasts than in growing plant organs while one growth-related gene is weakly expressed in protoplasts, as is the histone H4 gene. They seem to be induced in protoplasts by a synergistic effect of wounding and maceration. Sustained expression of the early genes is dependent on the presence of sucrose in the culture medium.
已分离出八个cDNA，其基因在生长阶段的悬浮细胞中比在静止期表达更强，且在成熟叶片中表达水平较低。相应的mRNA在幼嫩植物器官和萌发种子中大量积累，但在成熟植物组织和干种子中几乎检测不到。通过将核苷酸和蛋白质序列与EMBL和SWISSPROT数据库进行比较，对其中六个cDNA进行了表征。这八个与生长相关的基因在制备后不久（4小时）从烟草叶肉细胞分离的原生质体中表达。其中两个在新鲜分离的原生质体中表达（早期基因），而其他六个在培养4小时后检测到（晚期基因）。七个基因在原生质体中的表达比在生长的植物器官中更丰富，而一个与生长相关的基因在原生质体中表达较弱，组蛋白H4基因也是如此。它们似乎是由伤口和浸软的协同作用在原生质体中诱导产生的。早期基因的持续表达依赖于培养基中蔗糖的存在。
