Greber U F, Willetts M, Webster P, Helenius A
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
Cell. 1993 Nov 5;75(3):477-86. doi: 10.1016/0092-8674(93)90382-z.
Adenoviruses enter their host cells by receptor-mediated endocytosis and acid-activated penetration from endosomes into the cytosol and deliver their DNA genome into the nucleus. Our results show that incoming adenovirus type 2 particles undergo a stepwise disassembly program necessary to allow progress of the virus in the entry pathway and release of the genome into the nucleus. The fibers are released, the penton base structures dissociated, the proteins connecting the DNA to the inside surface of the capsid degraded or shed, and the capsid-stabilizing minor proteins eliminated. The uncoating process starts immediately upon endocytic uptake with the loss of fibers and ends with the uptake of dissociated hexon proteins and DNA into the nucleus.
腺病毒通过受体介导的内吞作用进入宿主细胞，并通过酸激活从内体穿透进入细胞质，然后将其DNA基因组传递到细胞核中。我们的结果表明，进入的2型腺病毒颗粒经历了一个逐步的拆解程序，这是病毒在进入途径中进展以及将基因组释放到细胞核所必需的。纤维被释放，五聚体基座结构解离，将DNA连接到衣壳内表面的蛋白质被降解或脱落，衣壳稳定的次要蛋白质被清除。脱壳过程在通过内吞作用摄取后立即随着纤维的丢失而开始，并以内吞解离的六邻体蛋白和DNA进入细胞核而结束。
