Kawaharajo K, Homma J Y
Jpn J Exp Med. 1975 Dec;45(6):515-24.
For the purpose of studying the pathogenesis of corneal infection of Pseudomonas aeruginosa, was examined virulence of elastase and protease producing strains or non-producing strains of the bacteria by using the cornea of mouse. The cornea of mouse was experimentally incised, and then P. aeruginosa cultures were dropped once onto it. As a result serious ulcers were caused over the entire cornea, and abscesses in its central area, by 10(5) and 10(7) viable cells of the enzymes producing strains IID 1210 and NO-5 of P. aeruginosa, respectively. Histological destruction, enlargement and cellular infiltration were observed in the corneal epithelium and stroma. On the other hand, P. aeruginosa strains NC-5 and N-10, enzymes non-producing ones, could not cause corneal lesions such as ulcers or abscesses. However, strain PA-103, which is considered to produce neither of the enzymes, could not cause corneal ulcers but cause uveitis even with 10(5) viable cells per mouse. Histologically, concentrated serous exudatation and severe cellular infiltration were noted in the anterior chamber. Necrosis was observed in the entire layer of the cornea. However, the corneal damage caused by strain PA-103 was clearly different from one due to the enzymes producing strains. Additive synergistic effect of protease on the virulent degree of strain NC-5 against the cornea was detected.
为了研究铜绿假单胞菌角膜感染的发病机制，利用小鼠角膜检测了该菌产弹性蛋白酶和蛋白酶菌株及不产酶菌株的毒力。实验性切开小鼠角膜，然后将铜绿假单胞菌培养物滴加在其上一次。结果，分别用铜绿假单胞菌产酶菌株IID 1210和NO - 5的10(5)和10(7)个活细胞接种后，整个角膜出现严重溃疡，中央区域出现脓肿。在角膜上皮和基质中观察到组织学破坏、肿大和细胞浸润。另一方面，不产酶的铜绿假单胞菌菌株NC - 5和N - 10不会引起角膜溃疡或脓肿等角膜病变。然而，被认为不产生这两种酶的菌株PA - 103，即使每只小鼠接种10(5)个活细胞，也不会引起角膜溃疡，但会引起葡萄膜炎。组织学上，在前房可见浆液性渗出物浓缩和严重的细胞浸润。在角膜全层观察到坏死。然而，菌株PA - 103引起的角膜损伤与产酶菌株引起的明显不同。检测到蛋白酶对菌株NC - 5对角膜的毒力程度有相加协同作用。
