Kawaharajo K, Homma J Y
Jpn J Exp Med. 1976 Jun;46(3):155-65.
Synergistic effects of immune gamma-globulin fraction containing antibodies of OPE, protease and elastase of Pseudomonas aeruginosa on the activities of antibiotic, dibekacin (DKB), in the cornea of mice were examined for the purpose of studying therapy for corneal ulcers due to Pseudomonal infection. In the case of the intramuscular injection, a medium effective dose (ED50) of DKB alone against corneal ulcers caused by strain IID 1210 of P. aeruginosa in experimental mice was 620 mug per mouse. When 15.6 to 18.7 mg of gamma-globulin fraction was subcutaneously given to each mouse prior to the infection with strain IID 1210, opacity instead of severe ulcers was observed only in the central area of cornea. The immune gamma-globulin fraction was far more effective in the protection of cornea from the infection than the calf serum that showed no antibody titer against OEP, protease and elastase. The ED50 values of DKB's combined with the immune gamma-globulin, Fr. 1 and Fr. 2, and the calf serum were 34 and 73, and 480 mug per mouse respectively. There was found no statistical difference in ED50 value between DKB combined with the calf serum and one without it. There was, however, significant difference in ED50 value between DKB's combined with Fr. 1 and Fr. 2, and one with the calf serum. When DKB alone is dropped in the eye of mouse for the protection, the ED50 value was 15 mug per mouse. When 1.56 to 1.87 mg of the immune gamma-globulin fraction was dropped in the eye after the infection with P. aeruginosa, there was observed no protection against corneal ulcers. the ED50 values of DKB's combined with Fr. 1, Fr. 2 and the calf serum were 0.96, 0.94 and 13 mug per mouse, respectively. There was no significant difference between the ED50 values of 0.96 and 0.94 mug, and between 15 and 18 mug. There was, however, significant difference between the former ED50 values (0.96 and 0.94 mug) and the latter ones (15 and 18 mug). The combination of DKB and the immune gamma-globulin fraction was found to be superior to the combination of DKB and the calf serum in the therapeutic effect on corneal ulcers caused by strain IID 1210 of P. aeruginosa.
为研究铜绿假单胞菌感染所致角膜溃疡的治疗方法，检测了含有抗铜绿假单胞菌外毒素、蛋白酶和弹性蛋白酶抗体的免疫球蛋白组分对小鼠角膜中抗生素地贝卡星（DKB）活性的协同作用。在肌肉注射的情况下，单独使用DKB对实验小鼠中由铜绿假单胞菌IID 1210菌株引起的角膜溃疡的半数有效剂量（ED50）为每只小鼠620微克。在用IID 1210菌株感染前，给每只小鼠皮下注射15.6至18.7毫克免疫球蛋白组分，仅在角膜中央区域观察到浑浊而非严重溃疡。免疫球蛋白组分在保护角膜免受感染方面比未检测到抗外毒素、蛋白酶和弹性蛋白酶抗体效价的小牛血清有效得多。DKB与免疫球蛋白组分Fr. 1、Fr. 2以及小牛血清联合使用时的ED50值分别为每只小鼠34微克、73微克和480微克。发现DKB与小牛血清联合使用和未联合使用时的ED50值无统计学差异。然而，DKB与Fr. 1、Fr. 2联合使用时的ED50值与和小牛血清联合使用时的ED50值存在显著差异。当单独将DKB滴眼用于保护时，ED50值为每只小鼠15微克。在用铜绿假单胞菌感染后滴眼1.56至1.87毫克免疫球蛋白组分时，未观察到对角膜溃疡的保护作用。DKB与Fr. 1、Fr. 2以及小牛血清联合使用时的ED50值分别为每只小鼠0.96微克、0.94微克和13微克。0.96微克和0.94微克的ED50值之间以及15微克和18微克的ED50值之间无显著差异。然而，前一组ED50值（0.96微克和0.94微克）与后一组（15微克和18微克）之间存在显著差异。发现在对铜绿假单胞菌IID 1210菌株引起的角膜溃疡的治疗效果上，DKB与免疫球蛋白组分的联合优于DKB与小牛血清的联合。
