Murata K
Research Institute for Food Science, Kyoto University, Japan.
Crit Rev Biotechnol. 1993;13(3):173-93. doi: 10.3109/07388559309041318.
Endospores of a bacterium Bacillus subtilis and ascospores of a yeast Saccharomyces cerevisiae contained almost all the activities for the same enzymes as vegetative cells. The biotechnological potential of spores was studied by selecting adenosine 5'-triphosphatase and alkaline phosphatase in bacterial and yeast spores, respectively, as model enzymes. The activity of both enzymes was efficiently expressed when the spores were treated by physical (sonication or electric field pulse) and chemical (organic solvents or detergents) methods. The yeast spores were immobilized in polyacrylamide gel without any appreciable loss of activity. The immobilized spores were packed in a column and used successfully for the continuous reactions of alkaline phosphatase and glyoxalase I. The microbial spores were confirmed to be promising as a biocatalyst for the production of useful chemicals in bioreactor systems.
枯草芽孢杆菌的内生孢子和酿酒酵母的子囊孢子所含的几乎所有相同酶的活性都与营养细胞相当。通过分别选择细菌和酵母孢子中的腺苷5'-三磷酸酶和碱性磷酸酶作为模型酶，研究了孢子的生物技术潜力。当孢子通过物理方法（超声处理或电场脉冲）和化学方法（有机溶剂或去污剂）处理时，这两种酶的活性都能有效表达。酵母孢子固定在聚丙烯酰胺凝胶中，活性没有明显损失。固定化的孢子被装填到柱子中，并成功用于碱性磷酸酶和乙二醛酶I的连续反应。微生物孢子被证实有望作为生物反应器系统中生产有用化学品的生物催化剂。
