März W, Siekmeier R, Scharnagl H, Seiffert U B, Gross W
Gustav Embden-Center of Biological Chemistry, Johann Wolfgang Goethe-University, Frankfurt/Main, Germany.
Clin Chem. 1993 Nov;39(11 Pt 1):2276-81.
Fast lipoprotein chromatography (FLPC) is a novel method for quantifying lipoproteins. Plasma proteins are separated by fast-flow gel filtration. Lipoproteins are detected by post-column derivatization with an enzymatic cholesterol reagent. FLPC resolves very-low-, low-, and high-density lipoproteins (VLDL, LDL, and HDL, respectively) and completely separates apolipoprotein Al- and apolipoprotein B-containing lipoproteins. CVs for VLDL-cholesterol, LDL-cholesterol, and HDL-cholesterol are 5.8%, 2.0%, and 1.9%, respectively. We compared FLPC with a combined ultracentrifugation and precipitation method and obtained correlation of r = 0.979, 0.978, and 0.933 for VLDL-cholesterol, LDL-cholesterol, and HDL-cholesterol, respectively. Triglyceride concentrations up to 9.00 g/L did not interfere with the quantification of lipoproteins by FLPC. We conclude that FLPC is a precise and reliable method for the analysis of plasma lipoproteins that complements conventional techniques.
快速脂蛋白色谱法（FLPC）是一种定量脂蛋白的新方法。血浆蛋白通过快速流动凝胶过滤进行分离。脂蛋白通过使用酶促胆固醇试剂进行柱后衍生化来检测。FLPC可分辨极低密度、低密度和高密度脂蛋白（分别为VLDL、LDL和HDL），并能完全分离含载脂蛋白A1和载脂蛋白B的脂蛋白。VLDL胆固醇、LDL胆固醇和HDL胆固醇的变异系数分别为5.8%、2.0%和1.9%。我们将FLPC与超速离心和沉淀联合法进行了比较，结果显示VLDL胆固醇、LDL胆固醇和HDL胆固醇的相关系数分别为r = 0.979、0.978和0.933。高达9.00 g/L的甘油三酯浓度不会干扰FLPC对脂蛋白的定量分析。我们得出结论，FLPC是一种精确可靠的血浆脂蛋白分析方法，可作为传统技术的补充。
