[快速脂蛋白色谱法：一种测定血浆脂蛋白的新方法]
[Fast lipoprotein chromatography: a new method for determining plasma lipoproteins].
作者信息
März W, Siekmeier R, Scharnagl H, Gross W
机构信息
Gustav-Embden-Zentrum der Biologischen Chemie, J.W. Goethe-Universität, Frankfurt am Main.
出版信息
Klin Wochenschr. 1990;68 Suppl 22:114-5.
Fast Lipoprotein Chromatography (FLPC) is a novel method for the quantitation of lipoprotein fractions. Plasma proteins are separated by fast flow gel filtration and lipoproteins are detected after post-column derivatization with an enzymatic cholesterol reagent. FLPC resolves VLDL, LDL, and HDL. Coefficients of variation for determinations of VLDL-, LDL-, and HDL-cholesterol are 5.8%, 2.0%, and 1.9%, respectively. Comparison of FLPC with a combined ultracentrifugation and precipitation method obtained correlation coefficients of 0.983, 0.973, and 0.941 for VLDL-C, LDL-C, and HDL-C, respectively.
快速脂蛋白色谱法（FLPC）是一种用于定量脂蛋白组分的新方法。血浆蛋白通过快速流动凝胶过滤进行分离，脂蛋白在柱后用酶促胆固醇试剂衍生化后进行检测。FLPC可分离极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）。测定VLDL-胆固醇、LDL-胆固醇和HDL-胆固醇的变异系数分别为5.8%、2.0%和1.9%。将FLPC与超速离心和沉淀联合方法进行比较，VLDL-C、LDL-C和HDL-C的相关系数分别为0.983、0.973和0.941。
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