Fielding B A, Humphreys S M, Allman R F, Frayn K N
Oxford Lipid Metabolism Group, Sheikh Rashid Laboratory, Radcliffe Infirmary, UK.
Clin Chim Acta. 1993 Jul 16;216(1-2):167-73. doi: 10.1016/0009-8981(93)90149-x.
A sensitive method has been developed to measure specific mono-, di- and triacylglycerol concentrations in human plasma, using thin-layer chromatography and enzymatic assay. The levels of partial acylglycerols in human plasma from fasting subjects were lower than previous reports had suggested and amounted to less than 3% of the total acylglycerols. After heparin injection the plasma monoacylglycerol concentration increased markedly (P < 0.01) while the triacylglycerol concentration decreased significantly (P < 0.001). The plasma diacylglycerol concentration did not change significantly although it increased as a percentage of the total (P < 0.05); after heparin partial acylglycerols accounted for more than 10% of the total. After a high-fat meal the plasma concentrations of di- and triacylglycerol increased approximately two-fold (P < 0.005) but no significant change was observed for mono-acylglycerol. The percentage contribution of partial acylglycerols was unchanged (2.6% fasting, 2.4% postprandially).
已开发出一种灵敏的方法，利用薄层色谱法和酶法测定人血浆中特定的单酰甘油、二酰甘油和三酰甘油浓度。空腹受试者血浆中部分酰基甘油的水平低于先前报告所提示的水平，占总酰基甘油的比例不到3%。注射肝素后，血浆单酰甘油浓度显著升高（P < 0.01），而三酰甘油浓度显著降低（P < 0.001）。血浆二酰甘油浓度虽作为总量的百分比有所增加，但未显著变化（P < 0.05）；注射肝素后，部分酰基甘油占总量的比例超过10%。高脂餐后，血浆中二酰甘油和三酰甘油的浓度增加了约两倍（P < 0.005），但单酰甘油未观察到显著变化。部分酰基甘油的贡献百分比未变（空腹时为2.6%，餐后为2.4%）。
