霍奇金病中里德-斯腾伯格细胞标志物restin的编码基因（RSN）定位于人类染色体12q24.3带及该基因座的酵母人工染色体克隆。

Localization of the gene (RSN) coding for restin, a marker for Reed-Sternberg cells in Hodgkin's disease, to human chromosome band 12q24.3 and YAC cloning of the locus.

作者信息

Hilliker C, Delabie J, Speleman F, Bilbe G, Bruggen J, Van Leuven F, Van den Berghe H

机构信息

Center for Human Genetics, Katholieke Universiteit van Leuven, Belgium.

出版信息

Cytogenet Cell Genet. 1994;65(3):172-6. doi: 10.1159/000133625.

DOI:10.1159/000133625

PMID:8222754

Abstract

A novel 160-kDa intermediate filament associated protein, named restin (Reed-Sternberg intermediate filament associated protein), is specifically expressed in the malignant cells of Hodgkin's disease and anaplastic large cell lymphoma (Ki-1 lymphoma). The combination of chromosomal R-banding and fluorescence in situ hybridization (FISH) with the use of two fluorescent dyes, fluorescein isothiocyanate and propidium iodide, allowed simultaneous detection of the hybridized DNA sequence and chromosomal R-banding. By this technique, the gene coding for restin (RSN) was assigned to chromosome region 12q24.31-->q24.33, while localization of the alpha-2-macroglobulin receptor (A2MR) was refined to 12q13.1-->q13.3. To further analyze the restin gene, a 500-kb YAC clone containing the gene was isolated and analyzed. A restriction map of this area is presented.

摘要

一种名为restin（里德-施特恩伯格中间丝相关蛋白）的新型160 kDa中间丝相关蛋白，在霍奇金病和间变性大细胞淋巴瘤（Ki-1淋巴瘤）的恶性细胞中特异性表达。利用异硫氰酸荧光素和碘化丙啶这两种荧光染料，将染色体R显带与荧光原位杂交（FISH）相结合，可同时检测杂交的DNA序列和染色体R显带。通过这项技术，编码restin（RSN）的基因被定位到染色体区域12q24.31→q24.33，而α-2-巨球蛋白受体（A2MR）的定位则精确到12q13.1→q13.3。为了进一步分析restin基因，分离并分析了一个包含该基因的500 kb酵母人工染色体（YAC）克隆。给出了该区域的限制性图谱。