Hilliker C, Delabie J, Speleman F, Bilbe G, Bruggen J, Van Leuven F, Van den Berghe H
Center for Human Genetics, Katholieke Universiteit van Leuven, Belgium.
Cytogenet Cell Genet. 1994;65(3):172-6. doi: 10.1159/000133625.
A novel 160-kDa intermediate filament associated protein, named restin (Reed-Sternberg intermediate filament associated protein), is specifically expressed in the malignant cells of Hodgkin's disease and anaplastic large cell lymphoma (Ki-1 lymphoma). The combination of chromosomal R-banding and fluorescence in situ hybridization (FISH) with the use of two fluorescent dyes, fluorescein isothiocyanate and propidium iodide, allowed simultaneous detection of the hybridized DNA sequence and chromosomal R-banding. By this technique, the gene coding for restin (RSN) was assigned to chromosome region 12q24.31-->q24.33, while localization of the alpha-2-macroglobulin receptor (A2MR) was refined to 12q13.1-->q13.3. To further analyze the restin gene, a 500-kb YAC clone containing the gene was isolated and analyzed. A restriction map of this area is presented.
一种名为restin（里德-施特恩伯格中间丝相关蛋白）的新型160 kDa中间丝相关蛋白，在霍奇金病和间变性大细胞淋巴瘤（Ki-1淋巴瘤）的恶性细胞中特异性表达。利用异硫氰酸荧光素和碘化丙啶这两种荧光染料，将染色体R显带与荧光原位杂交（FISH）相结合，可同时检测杂交的DNA序列和染色体R显带。通过这项技术，编码restin（RSN）的基因被定位到染色体区域12q24.31→q24.33，而α-2-巨球蛋白受体（A2MR）的定位则精确到12q13.1→q13.3。为了进一步分析restin基因，分离并分析了一个包含该基因的500 kb酵母人工染色体（YAC）克隆。给出了该区域的限制性图谱。
