Slim R, Machavoine C, Bernheim A
Laboratoire de Cytogénétique et de Génétique Oncologiques (CNRS URA 1158), Institut Gustave Roussy, Villejuif, France.
Cytogenet Cell Genet. 1994;65(1-2):104-7. doi: 10.1159/000133611.
Fluorescent in situ hybridization was used to assign 37 yeast artificial chromosomes (YACs) from the CEPH (Centre d'Etude du Polymorphisme Humain) libraries to R-banded metaphases stained with propidium iodide. The use of the whole yeast DNA as probe, allows rapid mapping and characterization of YAC clones. Of 37 YAC clones, 11 were found cytogenetically chimeric (30%). The YAC clones reported in this study can be used to isolate and characterize targeted regions involved in genetic diseases and cancer breakpoints.
采用荧光原位杂交技术，将来自CEPH（人类多态性研究中心）文库的37个酵母人工染色体（YAC）定位到用碘化丙啶染色的R带中期染色体上。使用完整的酵母DNA作为探针，可快速对YAC克隆进行定位和特征分析。在37个YAC克隆中，发现11个在细胞遗传学上是嵌合体（30%）。本研究报道的YAC克隆可用于分离和鉴定与遗传疾病及癌症断点相关的目标区域。
