两种从常规固定细胞中释放染色质用于荧光原位杂交的简单方法。
Two simple procedures for releasing chromatin from routinely fixed cells for fluorescence in situ hybridization.
作者信息
Fidlerová H, Senger G, Kost M, Sanseau P, Sheer D
机构信息
Human Cytogenetics and Human Immunogenetics Laboratories, Imperial Cancer Research Fund, London, UK.
出版信息
Cytogenet Cell Genet. 1994;65(3):203-5. doi: 10.1159/000133632.
PMID:8222761
Abstract
We describe two methods for releasing chromatin from routinely harvested and fixed cells. Using fluorescence in situ hybridization with combinations of probes from the HLA class II region, we show that good signals can be obtained on free chromatin fibers enabling determination of relationships between closely adjacent or overlapping probes.
摘要
我们描述了两种从常规收获和固定的细胞中释放染色质的方法。通过使用来自HLA II类区域的探针组合进行荧光原位杂交，我们发现在游离染色质纤维上可以获得良好的信号，从而能够确定紧密相邻或重叠探针之间的关系。
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