Fidlerová H, Senger G, Kost M, Sanseau P, Sheer D
Human Cytogenetics and Human Immunogenetics Laboratories, Imperial Cancer Research Fund, London, UK.
Cytogenet Cell Genet. 1994;65(3):203-5. doi: 10.1159/000133632.
We describe two methods for releasing chromatin from routinely harvested and fixed cells. Using fluorescence in situ hybridization with combinations of probes from the HLA class II region, we show that good signals can be obtained on free chromatin fibers enabling determination of relationships between closely adjacent or overlapping probes.
我们描述了两种从常规收获和固定的细胞中释放染色质的方法。通过使用来自HLA II类区域的探针组合进行荧光原位杂交，我们发现在游离染色质纤维上可以获得良好的信号，从而能够确定紧密相邻或重叠探针之间的关系。
