Lin Y Z
Department of Pathology, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1993 Mar;15(2):97-100.
The expression of C-myc and N-ras mRNA was studied in preneoplastic hepatic lesions induced by multiple small doses of diethylnitrosamine (DEN). Dot blot analysis showed that the expression of C-myc and N-ras mRNA was in elevated levels during the early stage of hepatocarcinogenesis. In situ hybridization in tissue sections demonstrated that the over-expression of C-myc and N-ras mRNA was clearly seen in some of the preneoplastic altered liver cell lesions. In comparison of these over-expressed oncogene lesions with the expression of carbamyl phosphate synthetase I (CPS I), known as a differentiation marker of hepatocytes, in serial sections revealed that almost all of the foci with over-expressed C-myc and/or N-ras mRNA also showed low expression of CPS I mRNA, which means a low differentiation nature of the liver cells. These findings suggested that the overexpression of C-myc and N-ras are closely correlated to the differentiation state of altered liver cells.
研究了多次小剂量二乙基亚硝胺（DEN）诱导的癌前肝损伤中C-myc和N-ras mRNA的表达。斑点印迹分析表明，在肝癌发生的早期阶段，C-myc和N-ras mRNA的表达水平升高。组织切片原位杂交显示，在一些癌前改变的肝细胞病变中可以清楚地看到C-myc和N-ras mRNA的过表达。将这些过表达的癌基因病变与作为肝细胞分化标志物的氨甲酰磷酸合成酶I（CPS I）在连续切片中的表达进行比较，发现几乎所有C-myc和/或N-ras mRNA过表达的病灶也显示CPS I mRNA低表达，这意味着肝细胞的低分化性质。这些发现表明，C-myc和N-ras的过表达与改变的肝细胞的分化状态密切相关。
