Kuhar S G, Feng L, Vidan S, Ross M E, Hatten M E, Heintz N
Department of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021-6399.
Development. 1993 Jan;117(1):97-104. doi: 10.1242/dev.117.1.97.
Among CNS neuronal populations, the cerebellar granule cell provides a simple model for analysing the molecular regulation of CNS neurogenesis. In this study, polyclonal antisera raised against immature granule cell precursors, purified from early postnatal mouse cerebellum, were used to isolate 39 unique cDNA clones from a lambda gt11 cDNA expression library made from the same cell population. Northern blot analysis revealed developmental stage and tissue-specific expression of 28 of the clones. In situ localization of mRNAs encoded by these novel cDNAs, as well as those encoding the axonal glycoprotein TAG-1 and the alpha 6 subunit of the GABAA receptor, reveal four distinct stages in cerebellar granule cell differentiation. The developmentally transient and spatially restricted expression of clones GC9 and GC44 identify a previously unrecognized step in cerebellar histogenesis.
在中枢神经系统神经元群体中，小脑颗粒细胞为分析中枢神经系统神经发生的分子调控提供了一个简单模型。在本研究中，用针对从出生后早期小鼠小脑中纯化的未成熟颗粒细胞前体产生的多克隆抗血清，从来自同一细胞群体构建的λgt11 cDNA表达文库中分离出39个独特的cDNA克隆。Northern印迹分析揭示了其中28个克隆的发育阶段和组织特异性表达。这些新cDNA编码的mRNA以及编码轴突糖蛋白TAG-1和GABAA受体α6亚基的mRNA的原位定位，揭示了小脑颗粒细胞分化的四个不同阶段。克隆GC9和GC44在发育过程中的短暂表达和空间限制表达，确定了小脑组织发生中一个以前未被认识的步骤。
