Tanaka M, Marunouchi T
Division of Cell Biology, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
Neurosci Lett. 1998 Feb 13;242(2):85-8. doi: 10.1016/s0304-3940(98)00032-9.
Some granule neurons naturally undergo apoptosis in the external granular layer (EGL) of the postnatally developing rat cerebellum. We analyzed the developmental stage-specificity of this apoptosis using double staining by in situ nick end labeling and immunohistochemistry against three proteins expressed at specific stages of granule neuron development. The amount of apoptosis of EGL neurons peaked on postnatal day 9. In the 9-day-old rat cerebellum, 54.0% of apoptotic EGL neurons expressed proliferating cell nuclear antigen. On the other hand, 22.2 and 15.4% of apoptotic EGL neurons existed in the postmitotic and premigratory zone defined by expression of TAG-1 and 440 kDa ankyrinB, respectively. Thus, proliferative granule neurons undergo apoptosis more frequently than postmitotic granule neurons in EGL of the developing cerebellum. This suggests that there are developmental stage-specific mechanisms of apoptosis of cerebellar granule neurons.
在出生后发育的大鼠小脑中，一些颗粒神经元会在外部颗粒层（EGL）自然发生凋亡。我们通过原位缺口末端标记和针对颗粒神经元发育特定阶段表达的三种蛋白质的免疫组织化学双重染色，分析了这种凋亡的发育阶段特异性。EGL神经元的凋亡量在出生后第9天达到峰值。在9日龄大鼠小脑中，54.0%的凋亡EGL神经元表达增殖细胞核抗原。另一方面，分别由TAG-1和440 kDa锚蛋白B的表达所定义的有丝分裂后区和迁移前区中，凋亡EGL神经元分别占22.2%和15.4%。因此，在发育中小脑的EGL中，增殖性颗粒神经元比有丝分裂后颗粒神经元更频繁地发生凋亡。这表明小脑颗粒神经元凋亡存在发育阶段特异性机制。
