Donovan G R, Baldo B A
Kolling Institute of Medical Research, Royal North Shore Hospital, St. Leonards, Australia.
Electrophoresis. 1993 Sep;14(9):917-22. doi: 10.1002/elps.11501401146.
IgE antibodies from sera having reactivity against ryegrass pollen protein allergens, wheat endosperm protein allergens and also several other cereal protein allergens were adsorbed with either ryegrass pollen or the wheat/globulin fraction immobilised on solid phases and subsequently eluted with low pH buffer. The eluted antibodies were reacted with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) blots of the different allergens. Antibodies adsorbed and subsequently eluted from the two allergen sources recognised different spectra of proteins in the ryegrass pollen and cereal allergen sources and indicated the degree of immunological cross-reactivity. Intra-species cross-reactivity of IgE antibodies was demonstrated employing similar methods to those used for the pollen and cereal allergens by using a recombinant allergen from the venom of the ant Myrmecia pilosula as the immunoadsorbent protein on the solid phase.
来自血清的IgE抗体对黑麦草花粉蛋白过敏原、小麦胚乳蛋白过敏原以及其他几种谷物蛋白过敏原具有反应性，这些抗体用固定在固相上的黑麦草花粉或小麦/球蛋白组分进行吸附，随后用低pH缓冲液洗脱。洗脱的抗体与不同过敏原的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）印迹反应。从两种过敏原来源吸附并随后洗脱的抗体识别出黑麦草花粉和谷物过敏原来源中不同的蛋白质谱，并表明了免疫交叉反应的程度。通过使用来自多毛蚁毒液的重组过敏原作为固相上的免疫吸附蛋白，采用与用于花粉和谷物过敏原的方法类似的方法，证明了IgE抗体的种内交叉反应。
