Sluyter R, Tovey E R, Duffy D L, Britton W J
Department of Medicine, University of Sydney, NSW, Australia.
Clin Exp Allergy. 1998 Mar;28(3):322-31. doi: 10.1046/j.1365-2222.1998.00223.x.
Both genetic and environmental factors are thought to contribute to specific IgE responses, however, the relative contribution of each in the responses to individual ryegrass pollen allergens is largely unknown even though some responses to allergens have been linked to certain HLA complexes.
Using a large group of monozygotic and dizygotic twins, this study was designed to investigate the IgE binding profiles of individual ryegrass pollen (Lolium perenne) components to assess the relative contribution of genetic and environmental factors in determining IgE responses to specific allergens.
Ryegrass pollen proteins were separated by electrophoresis and immunoblotted with sera from 191 pairs of twins where at least one sibling had a SPT > 2 mm to perennial ryegrass. Concordance levels for individual ryegrass pollen components were compared between monozygotic and dizygotic twins in a subset group where both twins had SPT > 3 mm to perennial ryegrass.
Immunoblotting revealed 23 individual IgE-binding components from ryegrass pollen. Although there was a significantly greater proportion of monozygotic twins with SPT wheals greater than 3 mm when compared with the dizygotic twins, the mean case-wise concordance for the immunoblot components was similar for both groups of twins. The mean case-wise concordance when at least four pairs of sera were involved was 44% for the MZ twins (n=11 components) and 45% for the DZ twins (n=12 components). We found no significant differences in concordance levels for any of the 23 individual components including allergens previously associated with HLA.
Evidence for genetic control of allergen-specific IgE responses in a large population sample of twins to individual ryegrass allergens is limited, indicating that the IgE responses to specific ryegrass pollen allergens are determined largely by environmental factors.
遗传因素和环境因素均被认为与特异性IgE反应有关，然而，尽管某些过敏原反应已与特定的HLA复合体相关联，但二者在对单个黑麦草花粉过敏原的反应中各自的相对贡献仍 largely unknown。
本研究使用一大组同卵双胞胎和异卵双胞胎，旨在调查单个黑麦草花粉（多年生黑麦草）成分的IgE结合谱，以评估遗传因素和环境因素在决定对特定过敏原的IgE反应中的相对贡献。
通过电泳分离黑麦草花粉蛋白，并用来自191对双胞胎的血清进行免疫印迹，其中至少有一个兄弟姐妹对多年生黑麦草的皮肤点刺试验（SPT）> 2 mm。在一个亚组中，比较了同卵双胞胎和异卵双胞胎对单个黑麦草花粉成分的一致性水平，该亚组中双胞胎对多年生黑麦草的SPT均> 3 mm。
免疫印迹显示黑麦草花粉中有23种个体IgE结合成分。尽管与异卵双胞胎相比，同卵双胞胎中SPT风团大于3 mm的比例明显更高，但两组双胞胎免疫印迹成分的平均病例一致性相似。当涉及至少四对血清时，同卵双胞胎（n = 11种成分）的平均病例一致性为44%，异卵双胞胎（n = 12种成分）为45%。我们发现，包括先前与HLA相关的过敏原在内的23种个体成分中的任何一种，其一致性水平均无显著差异。
在一大群双胞胎样本中，针对单个黑麦草过敏原的过敏原特异性IgE反应受遗传控制的证据有限，这表明对特定黑麦草花粉过敏原的IgE反应很大程度上由环境因素决定。
