Schwab K O, Bartels H, Martin C, Leichtenschlag E M
Department of Paediatrics, University of Würzburg, Germany.
Eur J Pediatr. 1993 Oct;152(10):797-801. doi: 10.1007/BF02073373.
Little is known about the receptor and post receptor mechanisms of sympathoadrenal signal transmission in type I diabetes mellitus. Therefore, we examined the maximum binding of granulocyte beta 2-adrenoceptors and the in vitro c-AMP accumulation in lymphocytes of 24 children and adolescents with diabetes mellitus and 14 similarly aged healthy subjects. The number of high affinity beta 2-adrenoceptors on granulocytes correlated significantly with unstimulated (r = 0.6, P < 0.004) and with isoproterenol stimulated c-AMP values in lymphocytes (r = 0.68, P < 0.0007) showing the proportional changes of beta 2-adrenoceptors and c-AMP in two different cells. The number of beta 2-adrenoceptors on granulocytes was significantly reduced in diabetic as compared to healthy children (median 1397, range 599-3405 vs. 2205, 825-3200 beta 2-adrenoceptors per granulocyte, P = 0.014). Moreover, the percentage in vitro stimulation of c-AMP by isoproterenol in lymphocytes was significantly reduced in diabetic children as compared to healthy individuals (120%, 39%-278% vs. 225%, 66%-500%, P = 0.012). These results indicate a decreased sympathoadrenergic signal transmission in peripheral blood cells as a model for the liver probably contributing to severe hypoglycaemia in diabetic children.
关于I型糖尿病中交感肾上腺信号传递的受体及受体后机制，人们了解甚少。因此，我们检测了24例糖尿病儿童和青少年以及14例年龄相仿的健康受试者的粒细胞β2 -肾上腺素能受体的最大结合力和淋巴细胞中体外c - AMP的积累情况。粒细胞上高亲和力β2 -肾上腺素能受体的数量与淋巴细胞中未受刺激的c - AMP值显著相关（r = 0.6，P < 0.004），也与异丙肾上腺素刺激后的c - AMP值显著相关（r = 0.68，P < 0.0007），这表明β2 -肾上腺素能受体和c - AMP在两种不同细胞中呈比例变化。与健康儿童相比，糖尿病儿童粒细胞上β2 -肾上腺素能受体的数量显著减少（中位数分别为每粒细胞1397个，范围599 - 3405个，以及每粒细胞2205个，范围825 - 3200个，P = 0.014）。此外，与健康个体相比，糖尿病儿童淋巴细胞中异丙肾上腺素对c - AMP的体外刺激百分比显著降低（分别为120%，范围39% - 278%，以及225%，范围66% - 500%，P = 0.012）。这些结果表明，作为肝脏模型的外周血细胞中交感肾上腺素能信号传递减少，这可能是导致糖尿病儿童严重低血糖的原因之一。
