Nijs G, De Witte P, Geboes K, Mertens J, Eyssen H
Laboratory of Pharmaceutical Biology and Phytopharmacology, Institute of Pharmaceutical Sciences, K.U. Leuven, Belgium.
Eur J Pharmacol. 1993 Aug 3;239(1-3):241-3. doi: 10.1016/0014-2999(93)91003-6.
This study shows that rhein anthrone has no laxative potency in germ-free rats because after intracaecal administration of a dose of 50 mg/kg the large intestine transit exceeded 240 min. The time course of the laxative potency of rhein anthrone injected intracaecally was evaluated after peroral inoculation of germ-free rats with the caecal contents of conventional rats. Large intestine transit was measured at consecutive periods, on days 0, 1, 2, 3 and 5 after peroral inoculation. It appeared that 1 day after peroral inoculation the laxative potency of rhein anthrone was already established (large intestinal transit < 10 min) and laxation remained on the following days (days 2, 3 and 5). We concluded that rhein anthrone is inactive in germ-free rats and acquires laxative potency after peroral inoculation of germ-free rats with caecal contents of conventional rats.
本研究表明，大黄素蒽酮对无菌大鼠没有致泻作用，因为在盲肠内给予50mg/kg的剂量后，大肠转运时间超过了240分钟。在用常规大鼠的盲肠内容物经口接种无菌大鼠后，评估了盲肠内注射大黄素蒽酮的致泻作用的时间进程。在经口接种后的第0、1、2、3和5天连续测量大肠转运时间。结果显示，经口接种1天后，大黄素蒽酮的致泻作用已经确立（大肠转运时间<10分钟），并且在接下来的几天（第2、3和5天）仍保持通便作用。我们得出结论，大黄素蒽酮在无菌大鼠中无活性，在用常规大鼠的盲肠内容物经口接种无菌大鼠后获得致泻作用。
