Galantsev V P, Kovalenko S G, Moltchanov A A, Prutskov V I
A. A. Ukhtomsky Institute of Physiology, St. Petersburg State University, Russia.
Experientia. 1993 Oct 15;49(10):870-5. doi: 10.1007/BF01952600.
In mammary explants of lactating mice, changes in the intensity of chemiluminescence (CL) were observed after the addition to the incubation medium of hormones and mediators that are involved in the regulation of secretion: oxytocin, acetylcholine, epinephrine and norepinephrine. A 15-min period of treatment with oxytocin, epinephrine or norepinephrine changed the level of thiobarbituric acid-reactive substances (TBARS). Two mammary explants, one of which was treated with oxytocin, acetylcholine, epinephrine or norepinephrine, were found to interact even when separated by a quartz glass wall. Analysis of the level of TBARS formation in these two explants showed that the observed interactions might be connected with light emission resulting from lipid peroxidation (LP) processes. The possible role of LP and low-level CL in the regulation of mammary gland secretion is discussed.
在泌乳小鼠的乳腺外植体中，向孵育培养基中添加参与分泌调节的激素和介质（催产素、乙酰胆碱、肾上腺素和去甲肾上腺素）后，观察到化学发光（CL）强度的变化。用催产素、肾上腺素或去甲肾上腺素处理15分钟会改变硫代巴比妥酸反应性物质（TBARS）的水平。发现两个乳腺外植体，其中一个用催产素、乙酰胆碱、肾上腺素或去甲肾上腺素处理，即使被石英玻璃壁隔开也会相互作用。对这两个外植体中TBARS形成水平的分析表明，观察到的相互作用可能与脂质过氧化（LP）过程产生的发光有关。讨论了LP和低水平CL在乳腺分泌调节中的可能作用。
