Oberholzer T, Mantei N, Semenza G
Department of Biochemistry, Swiss Federal Institute of Technology, ETH-Zentrum, Zürich.
FEBS Lett. 1993 Oct 25;333(1-2):127-31. doi: 10.1016/0014-5793(93)80389-c.
Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro sequence and the lactase domain of mature LPH, accumulated intracellularly; the pro sequence with no mature domain was secreted. We conclude that the pro sequence is important for LPH to be transported to the cell surface.
将编码人前-前乳糖酶-根皮苷水解酶（pre-proLPH）部分或全部的各种cDNA转染到COS细胞中，并评估乳糖酶相关蛋白的亚细胞定位。只有完整的proLPH到达质膜。没有pro序列的LPH以及包含pro序列和成熟LPH乳糖酶结构域的构建体在细胞内积累；没有成熟结构域的pro序列被分泌。我们得出结论，pro序列对于LPH转运到细胞表面很重要。
