从鳟鱼血浆中纯化一种与赖氨酰缓激肽相关的血管活性肽。

Purification of a vasoactive peptide related to lysyl-bradykinin from trout plasma.

作者信息

Conlon J M, Olson K R

机构信息

Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178.

出版信息

FEBS Lett. 1993 Nov 8;334(1):75-8. doi: 10.1016/0014-5793(93)81684-r.

DOI:10.1016/0014-5793(93)81684-r

PMID:8224232

Abstract

Incubation of plasma from the steelhead trout, Oncorhynchus mykiss with porcine pancreatic glandular kallikrein generated bradykinin-like immunoreactivity. The primary structure of the immunoreactive peptide was established as: Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg. This sequence shows two amino acid substitutions (Phe6-->Trp and Phe9-->Leu) compared with mammalian lysyl-bradykinin (kallidin). Bolus intraarterial injection of the purified peptide produced a strong and sustained vasopressor response in the unanaesthetized trout. The data demonstrate that the kallikrein-kinin system predates the appearance of tetrapods and suggest a role for this system in cardiovascular regulation in fish.

摘要

将虹鳟鱼（Oncorhynchus mykiss）的血浆与猪胰腺腺体激肽释放酶一起孵育，产生了类缓激肽免疫反应性。免疫反应性肽的一级结构确定为：Lys-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg。与哺乳动物赖氨酰缓激肽（胰激肽）相比，该序列显示出两个氨基酸取代（Phe6→Trp和Phe9→Leu）。在未麻醉的鳟鱼中，经动脉推注纯化的肽会产生强烈且持续的血管升压反应。数据表明，激肽释放酶-激肽系统早于四足动物出现，并表明该系统在鱼类心血管调节中发挥作用。