激肽释放酶从人血浆蛋白中释放出的[羟脯氨酸3]-赖氨酰缓激肽的鉴定。

Identification of [hydroxyproline3]-lysyl-bradykinin released from human plasma protein by kallikrein.

作者信息

Sasaguri M, Ikeda M, Ideishi M, Arakawa K

机构信息

Department of Internal Medicine, Fukuoka University, School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Jan 15;150(1):511-6. doi: 10.1016/0006-291x(88)90550-5.

DOI:10.1016/0006-291x(88)90550-5

PMID:3337729

Abstract

[Hydroxyproline3]-lysyl-bradykinin [( Hyp3]-Lys-BK), a new kinin was isolated, besides lysyl-bradykinin (Lys-BK), from the reaction mixture of human plasma protein Cohn's fraction IV-4 with hog pancreatic kallikrein. The liberated kinins were isolated by procedures including ethanol extraction, Sephadex G-15, CM cellulose and reverse-phase high performance liquid chromatography (HPLC) and quantitated by radioimmunoassay. On HPLC, two peaks of immunoreactive kinins emerged. Peak 1, an unknown kinin proceeded to Peak 2 which had an identical retention time to that of Lys-BK. The amino acid sequence of the unknown Peak 1 proved to be Lys-Arg-Pro-Hyp-Gly-Phe-Ser-Pro-Phe-Arg, or [Hyp3]-Lys-BK, and Peak 2 Lys-BK. The ratio of the amounts of two kinins thus formed were [Hyp3]-Lys-BK 25 +/- 4% and Lys-BK 75 +/- 4%. The existence of [Hyp3]-Lys-BK suggests a presence of a new kininogen, containing [Hyp3]-Lys-BK in human plasma protein, possibly undergone post-translational modifications.

摘要

除了赖氨酰缓激肽（Lys-BK）之外，还从人血浆蛋白科恩氏IV-4组分与猪胰激肽释放酶的反应混合物中分离出一种新的激肽——[羟脯氨酸3]-赖氨酰缓激肽（[(Hyp3]-Lys-BK）。通过包括乙醇萃取、葡聚糖凝胶G-15、CM纤维素和反相高效液相色谱（HPLC）在内的方法分离出释放的激肽，并通过放射免疫测定法定量。在HPLC上，出现了两个免疫反应性激肽峰。峰1，一种未知激肽转化为峰2，其保留时间与Lys-BK相同。未知峰1的氨基酸序列被证明是Lys-Arg-Pro-Hyp-Gly-Phe-Ser-Pro-Phe-Arg，即[Hyp3]-Lys-BK，峰2是Lys-BK。如此形成的两种激肽的量的比例为[Hyp3]-Lys-BK 25±4%和Lys-BK 75±4%。[Hyp3]-Lys-BK的存在表明人血浆蛋白中存在一种新的激肽原，其含有[Hyp3]-Lys-BK，可能经历了翻译后修饰。