使用DNA探针检测环境样本中的福氏耐格里阿米巴包囊。
Detection of Naegleria fowleri cysts in environmental samples by using a DNA probe.
作者信息
Sparagano O
机构信息
Département d'Hygiène Appliquée, Institut Pasteur de Lyon, France.
出版信息
FEMS Microbiol Lett. 1993 Sep 15;112(3):349-51. doi: 10.1111/j.1574-6968.1993.tb06474.x.
In this study we tried to detect DNA Naegleria fowleri in artificially contaminated environmental samples, with or without sediments, containing 10(4) cysts of this pathogenic amoeba. We used two assays to extract DNA from samples: first, direct DNA extraction, which gave positive results only for water samples without sediment; second, DNA extraction after sample incubation on agar plates, which allowed us to remove amoeba growing out of the sediments, and which gave positive results for all samples, even those initially with sediments (5, 500 or 500 mg). Thus, this molecular identification appears as a powerful tool to investigate N. fowleri growth in environmental samples.
在本研究中，我们试图从含有10⁴个致病性福氏耐格里阿米巴囊肿的人工污染环境样本（有沉积物或无沉积物）中检测福氏耐格里阿米巴的DNA。我们使用两种方法从样本中提取DNA：第一种是直接DNA提取法，该方法仅对无沉积物的水样得出阳性结果；第二种是在琼脂平板上孵育样本后进行DNA提取，这种方法使我们能够去除从沉积物中生长出来的阿米巴，并且对所有样本（即使是最初含有5mg、50mg或500mg沉积物的样本）都得出了阳性结果。因此，这种分子鉴定似乎是研究环境样本中福氏耐格里阿米巴生长情况的有力工具。
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