一种用于从环境中鉴定福氏耐格里阿米巴的聚合酶链式反应（PCR）的开发。
Development of a PCR for identification of Naegleria fowleri from the environment.
作者信息
Kilvington S, Beeching J
机构信息
Public Health Laboratory, Royal United Hospital, Combe Park, Bath, England.
出版信息
Appl Environ Microbiol. 1995 Oct;61(10):3764-7. doi: 10.1128/aem.61.10.3764-3767.1995.
Abstract
A species-specific PCR for the identification of Naegleria fowleri was developed. In sensitivity studies, 10 trophozoites or cysts and 1 trophozoite or cyst could be detected after 35 and 45 cycles, respectively. In conjunction with a rapid DNA isolation method, this PCR was used to identify N. fowleri directly from primary cultures of environmental samples.
摘要
开发了一种用于鉴定福氏耐格里阿米巴的种特异性聚合酶链反应（PCR）。在敏感性研究中，分别经过35个和45个循环后，可检测到10个滋养体或包囊以及1个滋养体或包囊。结合快速DNA提取方法，该PCR被用于直接从环境样本的原代培养物中鉴定福氏耐格里阿米巴。
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7
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