人类核糖体蛋白L37a：cDNA的克隆及在组织和细胞系中的差异基因表达分析

Human ribosomal protein L37a: cloning of the cDNA and analysis of differential gene expression in tissues and cell lines.

作者信息

Saha D P, Tirumalai P S, Scala L A, Howells R D

机构信息

Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School 07103.

出版信息

Gene. 1993 Oct 15;132(2):285-9. doi: 10.1016/0378-1119(93)90209-l.

DOI:10.1016/0378-1119(93)90209-l

PMID:8224875

Abstract

A cDNA corresponding to human ribosomal protein L37a (hL37a) was obtained by screening a SHSY5Y neuroblastoma library. The full-length cDNA contained 366 nucleotides (nt) in addition to a poly(A) tail. The pyrimidine-rich sequence, CTTTCT, that is common to many ribosomal protein-encoding cDNAs was present at the 5' terminus. The nt sequence displayed 85% identity with rat L37a (rL37a) cDNA. The predicted protein contains 92 amino acids with a M(r) of 10,277, is highly basic, and has 100% sequence identity with rL37a. A putative zinc-finger domain is present in the central region of the protein. Human lymphocytes and several human cell lines express hL37a mRNA at significantly higher levels than the rat cell lines and rat tissues tested. The hL37a gene does not contain introns.

摘要

通过筛选SHSY5Y神经母细胞瘤文库获得了与人核糖体蛋白L37a（hL37a）对应的cDNA。除了聚腺苷酸尾外，全长cDNA包含366个核苷酸（nt）。许多核糖体蛋白编码cDNA共有的富含嘧啶的序列CTTTCT存在于5'末端。该核苷酸序列与大鼠L37a（rL37a）cDNA显示出85%的同一性。预测的蛋白质包含92个氨基酸，分子量为10277，具有高度碱性，并且与rL37a具有100%的序列同一性。在该蛋白质的中央区域存在一个假定的锌指结构域。人淋巴细胞和几种人细胞系表达hL37a mRNA的水平明显高于所测试的大鼠细胞系和大鼠组织。hL37a基因不含内含子。