编码核糖体蛋白L12和L18的小鼠cDNA的序列分析。

Sequence analysis of mouse cDNAs encoding ribosomal proteins L12 and L18.

作者信息

Hou E W, Li S S

机构信息

Laboratory of Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709.

出版信息

Gene. 1993 Aug 25;130(2):287-90. doi: 10.1016/0378-1119(93)90433-4.

DOI:10.1016/0378-1119(93)90433-4

PMID:8359697

Abstract

Mouse cDNAs encoding ribosomal proteins (r-proteins), L12 and L18, were isolated and their sequences determined. The L12 cDNA was found to contain 639 bp, including a coding sequence of 498 nucleotides (nt), 5' (78 nt) and 3' (45 nt) untranslated regions (UTRs), and a poly(A) tail of 18 nt. The L18 cDNA was shown to consist of 648 bp, including a coding sequence of 567 nt, 5' (26 nt) and 3' (39 nt) UTRs, and a poly(A) tail of 16 nt. The nt sequences of the protein-coding region from the mouse L12 and L18 cDNAs were found to exhibit 96% and 92% identity, respectively, with those of the rat. With the use of mouse L12 and L18 cDNA probes, multiple (at least 10) copies of the L12 and L18 gene families were shown to be present in the mouse and rat genomes. However, there was no sequence heterogeneity detected among seven L18 cDNA clones, indicating that only one copy of the L18 gene-related sequences is functional, and the other copies are presumably nonfunctional pseudogenes. The complete amino acid (aa) sequences of the mouse r-proteins, L12 and L18, were deduced from the nt sequences of their cDNA clones. L12 has 165 aa and a M(r) of 17,790, while L18 has 188 aa and a M(r) of 21,570. The aa sequences of the mouse r-proteins, L12 and L18, exhibit 98% and 94% identity, respectively, to those of rat.

摘要

分离出编码核糖体蛋白（r-蛋白）L12和L18的小鼠cDNA，并测定了它们的序列。发现L12 cDNA包含639个碱基对（bp），包括一个498个核苷酸（nt）的编码序列、5'（78 nt）和3'（45 nt）非翻译区（UTR）以及一个18 nt的聚腺苷酸（poly(A)）尾。L18 cDNA显示由648 bp组成，包括一个567 nt的编码序列、5'（26 nt）和3'（39 nt）UTR以及一个16 nt的poly(A)尾。发现小鼠L12和L18 cDNA的蛋白质编码区的核苷酸序列与大鼠的分别具有96%和92%的同一性。使用小鼠L12和L18 cDNA探针，显示小鼠和大鼠基因组中存在多个（至少10个）L12和L18基因家族拷贝。然而，在七个L18 cDNA克隆中未检测到序列异质性，表明L18基因相关序列只有一个拷贝是有功能的，其他拷贝可能是无功能的假基因。从小鼠r-蛋白L12和L18的cDNA克隆的核苷酸序列推导出它们完整的氨基酸（aa）序列。L12有165个氨基酸，相对分子质量（M(r)）为17,790，而L18有188个氨基酸，M(r)为21,570。小鼠r-蛋白L12和L18的氨基酸序列与大鼠的分别具有98%和94%的同一性。