α-生育酚自由基与生物及药理活性物质的反应动力学

Reaction kinetics of alpha-tocopheroxyl radical with biologically and pharmacologically active substances.

作者信息

Ondrias K, Misík V, Brezová V, Stasko A

机构信息

Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava.

出版信息

Free Radic Res Commun. 1993;19(1):17-28. doi: 10.3109/10715769309056495.

DOI:10.3109/10715769309056495

PMID:8225031

Abstract

The alpha-tocopheroxyl radical (alpha TR.)generated in the reaction with 1,1-diphenyl-2-picrylhydrazyl in n-butanol decayed according to second-order kinetics with a rate constant k alpha = 3 x 10(3) M-1s-1 as determined by EPR spectroscopy. Various biologically and pharmacologically active substances like isoprenaline (ISO), epinephrine (EPI), histamine (HIS), stobadine (STO), nafazatrom (NAF) and Kampo C medicine (KMC) accelerated the decay rate of alpha TR(.). The whole process is formally a third-order reaction with the rate constants (in 10(9) M-2s-1): ks(ISO) = 1.28, ks(NAF) = 1.25, ks(EPI) = 0.6, ks(HIS) = 0.4, and ks(STO) = 0.1. In the kinetics of the reaction mechanism, bimolecular intermediates are assumed and the rate constants of their formation were determined.

摘要

在正丁醇中，与1,1 - 二苯基 - 2 - 苦基肼反应生成的α - 生育酚氧基自由基（αTR.）按照二级动力学衰减，通过电子顺磁共振光谱法测定其速率常数kα = 3×10³ M⁻¹s⁻¹。各种生物和药理活性物质，如异丙肾上腺素（ISO）、肾上腺素（EPI）、组胺（HIS）、司他定（STO）、萘呋胺酯（NAF）和汉方C药（KMC）加速了αTR(.)的衰减速率。整个过程形式上是一个三级反应，其速率常数（单位为10⁹ M⁻²s⁻¹）：ks(ISO) = 1.28，ks(NAF) = 1.25，ks(EPI) = 0.6，ks(HIS) = 0.4，ks(STO) = 0.1。在反应机理的动力学中，假定存在双分子中间体并测定了它们的形成速率常数。