Ben-Efraim S, Tak C, Fieren M J, Romijn J C, Beckmann I, Bonta I L
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
Immunol Lett. 1993 Jul;37(1):27-33. doi: 10.1016/0165-2478(93)90128-o.
Human peritoneal macrophages collected from renal patients on continuous ambulatory peritoneal dialysis (CAPD) during inflammation-free periods were induced to express antitumor activity in vitro when cultured in the presence of bacterial lipopolysaccharide (LPS) and even more activity when they were kept in the presence of LPS + IND (indomethacin). The antitumor activity was expressed against a human tumor-cell line, RC43, either in a cell-to-cell contact set-up between the macrophages and the RC43 target cells or when the tumor cells were exposed to supernatants of the cultured macrophages. The antitumor activity of macrophages was correlated to a marked increase in production of tumor necrosis factor-alpha (TNF alpha), not correlated to an increase in nitrite production and inversely correlated to the production of PGE2. The RC43 tumor cells were susceptible to recombinant human TNF alpha, recombinant human IL-1 beta, sodium nitrite and the leukotriene LTB4. The results obtained suggest that activated human macrophages might represent a useful tool for cancer immunotherapy.
在无炎症期从持续非卧床腹膜透析（CAPD）的肾病患者体内收集的人腹膜巨噬细胞，当在细菌脂多糖（LPS）存在的情况下培养时，可在体外被诱导表达抗肿瘤活性，而当它们在LPS + 吲哚美辛（IND）存在的情况下培养时，抗肿瘤活性更强。这种抗肿瘤活性针对人肿瘤细胞系RC43表现出来，无论是在巨噬细胞与RC43靶细胞之间的细胞间接触设置中，还是当肿瘤细胞暴露于培养的巨噬细胞的上清液时。巨噬细胞的抗肿瘤活性与肿瘤坏死因子-α（TNFα）产生的显著增加相关，与亚硝酸盐产生的增加无关，且与前列腺素E2的产生呈负相关。RC43肿瘤细胞对重组人TNFα、重组人白细胞介素-1β、亚硝酸钠和白三烯LTB4敏感。所获得的结果表明，活化的人巨噬细胞可能是癌症免疫治疗的一种有用工具。
