Turner S J, Carbone F R, Strugnell R A
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Infect Immun. 1993 Dec;61(12):5374-80. doi: 10.1128/iai.61.12.5374-5380.1993.
Recombinant Salmonella typhimurium aroA aroD mutants which expressed ovalbumin were constructed. The two expression constructs used were based on either pUC18 or pBR322. The pBR322-based construct was more stable in vitro and in vivo than the pUC-based construct. Salmonellae containing the stable pBR322-based plasmid induced major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTL), in contrast to salmonellae containing the pUC18-based expression construct. The priming of MHC class I-restricted CTL was increased by multiple immunizations. The study described in this report suggest that S. typhimurium delta aro mutants have the capacity to induce MHC class I-restricted CTL against carried antigens and that MHC class I-restricted CTL responses require stable in vivo expression of the target antigen. Further, the results indicate that the Salmonella typhi delta aro mutants currently undergoing evaluation in studies with humans may be good carriers of viral antigens with CTL determinants.
构建了表达卵清蛋白的重组鼠伤寒沙门氏菌aroA aroD突变体。所使用的两种表达构建体分别基于pUC18或pBR322。与基于pUC的构建体相比，基于pBR322的构建体在体外和体内都更稳定。与含有基于pUC18的表达构建体的沙门氏菌相比，含有稳定的基于pBR322的质粒的沙门氏菌诱导了主要组织相容性复合体（MHC）I类限制性细胞毒性T淋巴细胞（CTL）。多次免疫可增强MHC I类限制性CTL的致敏作用。本报告中描述的研究表明，鼠伤寒沙门氏菌aro突变体有能力诱导针对携带抗原的MHC I类限制性CTL，且MHC I类限制性CTL反应需要靶抗原在体内稳定表达。此外，结果表明，目前正在人体研究中进行评估的伤寒沙门氏菌aro突变体可能是具有CTL决定簇的病毒抗原的良好载体。
