Marks N J, Halton D W, Shaw C, Johnston C F
Comparative Neuroendocrinology Research Group, School of Biology & Biochemistry, Queen's University of Belfast, Northern Ireland, U.K.
Int J Parasitol. 1993 Aug;23(5):617-25. doi: 10.1016/0020-7519(93)90168-x.
The localization and distribution of cholinergic, serotoninergic and peptidergic nerve elements in the proteocephalidean tapeworm, Proteocephalus pollanicola, have been investigated by enzyme histochemistry, and by an indirect immunofluorescence technique interfaced with confocal scanning laser microscopy. Cholinesterase (ChE) activity was localized in the major components of the central nervous system (CNS) and the peripheral nervous system (PNS), including the innervation of the reproductive structures of the worm. Serotoninergic (5-HT) nerves were found in the paired cerebral ganglia, transverse commissure and in the 10 longitudinal nerve cords. Antisera to 17 mammalian regulatory peptides and the invertebrate peptide FMRFamide have been used to explore the peptidergic nervous system of the worm. The most extensive immunostaining occurred with antisera raised to members of the neuropeptide Y superfamily, namely neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP). In all cases, intense immunoreactivity was found in numerous cell bodies and fibres of both the CNS and PNS, including the innervation of the reproductive apparatus. FMRFamide antisera stained the same structures to a comparable degree as those raised to the NPY superfamily. Cholinergic and peptidergic elements were much more prevalent within the CNS, while the serotoninergic nerve fibres tended to dominate in the PNS. The overlap obtained in staining patterns for the peptidergic and cholinergic components suggests that there may be a certain amount of co-localization of peptides with small-molecule transmitter substances in the same neurone. Weak staining for the tachykinin, substance P and for calcitonin gene-related peptide (CGRP) was confined to the major longitudinal nerve cords.
通过酶组织化学以及与共聚焦扫描激光显微镜联用的间接免疫荧光技术，对Pollanicola头槽绦虫中胆碱能、5-羟色胺能和肽能神经成分的定位与分布进行了研究。胆碱酯酶（ChE）活性定位于中枢神经系统（CNS）和外周神经系统（PNS）的主要组成部分，包括该蠕虫生殖结构的神经支配。在成对的脑神经节、横向连合以及10条纵神经索中发现了5-羟色胺能（5-HT）神经。已使用针对17种哺乳动物调节肽和无脊椎动物肽FMRF酰胺的抗血清来探索该蠕虫的肽能神经系统。对神经肽Y超家族成员，即神经肽Y（NPY）、肽YY（PYY）和胰多肽（PP）产生的抗血清出现了最广泛的免疫染色。在所有情况下，在CNS和PNS的许多细胞体和纤维中都发现了强烈的免疫反应性，包括生殖器官的神经支配。FMRF酰胺抗血清对相同结构的染色程度与针对NPY超家族产生的抗血清相当。胆碱能和肽能成分在CNS中更为普遍，而5-羟色胺能神经纤维在PNS中往往占主导地位。肽能和胆碱能成分染色模式的重叠表明，在同一神经元中，肽与小分子递质物质可能存在一定程度的共定位。速激肽、P物质和降钙素基因相关肽（CGRP）的弱染色仅限于主要的纵神经索。
