Garofalo A, Chirivi R G, Scanziani E, Mayo J G, Vecchi A, Giavazzi R
Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
Invasion Metastasis. 1993;13(2):82-91.
The growth and metastatic behavior of human tumor cell lines were studied in nude, beige/nude/xid (bg/nu/xid) and severe combined immunodeficient (SCID) mice. One melanoma, two colon carcinomas and one renal carcinoma grew subcutaneously in the three strains of mice with no significant differences in tumor take and growth rate. One ovarian carcinoma formed a subcutaneously growing tumor only in SCID mice. Spontaneous metastases were observed only in mice with advanced subcutaneous tumors or at histological examination, but more frequently in bg/nu/xid and SCID mice than in nude mice. A375M melanoma formed more lung colonies in nude and bg/nu/xid mice than in SCID mice. HT-29LM colon carcinoma injected intravenously or intrasplenically formed more lung and liver colonies and lymph node metastases in bg/nu/xid mice than in nude and SCID mice. The treatment of SCID mice with anti-asGM1 serum depleted NK activity and enhanced the number of lung colonies by A375M melanoma and HT-29LM colon carcinoma. Bg/nu/xid or SCID mice may therefore offer some advantages for studying the malignant behavior of human solid tumors and differences may depend on the experimental conditions and on the tumor type.
在裸鼠、米色/裸鼠/ xid（bg/nu/xid）小鼠和严重联合免疫缺陷（SCID）小鼠中研究了人肿瘤细胞系的生长和转移行为。一种黑色素瘤、两种结肠癌和一种肾癌在这三种小鼠品系中均可皮下生长，肿瘤接种率和生长速率无显著差异。一种卵巢癌仅在SCID小鼠中形成皮下生长的肿瘤。仅在皮下肿瘤晚期的小鼠或组织学检查时观察到自发转移，但在bg/nu/xid和SCID小鼠中比在裸鼠中更频繁。A375M黑色素瘤在裸鼠和bg/nu/xid小鼠中形成的肺集落比在SCID小鼠中更多。静脉内或脾内注射HT-29LM结肠癌在bg/nu/xid小鼠中形成的肺和肝集落以及淋巴结转移比在裸鼠和SCID小鼠中更多。用抗asGM1血清处理SCID小鼠可耗尽NK活性，并增加A375M黑色素瘤和HT-29LM结肠癌的肺集落数量。因此，bg/nu/xid或SCID小鼠可能为研究人类实体瘤的恶性行为提供一些优势，差异可能取决于实验条件和肿瘤类型。
