Miyazawa T, Murayama C, Yamanaka T, Fritz-Zieroth B
Nihon Schering K.K., Research Department, Osaka, Japan.
Invest Radiol. 1993 Sep;28(9):831-7.
Hypertonic X-ray contrast media induce morphologic changes in red blood cells (RBCs) and reduce their deformability when measured in vitro. This study investigated whether RBCs treated with hypertonic contrast media could induce circulatory as well as metabolic disturbances in vivo.
Autologous blood was mixed with an equal volume of meglumine diatrizoate (306 mgI/mL); 50 microliters of this mixture was infused into the left internal carotid artery of rats 2 minutes after mixing. Five minutes after infusion, the cerebral blood flow was determined using iodo[14C]antipyrine. 31P-magnetic resonance spectroscopy (MRS), 1H-MRS and 1H-magnetic resonance imaging (MRI) of the brain were performed before and after infusion of the mixture to study changes in energy metabolites and the integrity of the blood-brain barrier (BBB), as well as the development of edema.
The blood flow decreased by 70% to 80% in the left cerebral cortex and caudate-putamen. 31P-MRS and 1H-MRS demonstrated derangement of oxidative phosphorylation. 1H-MRI demonstrated instant destruction of the BBB and gradual progress of edema in the left hemisphere.
These results indicate that intracarotid infusion of RBCs treated with hypertonic contrast medium can induce embolization and subsequent ischemic damage to the brain.
高渗X线造影剂可在体外使红细胞（RBC）形态改变并降低其变形能力。本研究旨在探究经高渗造影剂处理的红细胞在体内是否会引发循环及代谢紊乱。
将自体血与等量的泛影葡胺（306 mgI/mL）混合；混合2分钟后，将50微升此混合物注入大鼠左颈内动脉。注入后5分钟，使用碘[14C]安替比林测定脑血流量。在注入混合物前后进行脑部的31P磁共振波谱（MRS）、1H-MRS和1H磁共振成像（MRI），以研究能量代谢物的变化、血脑屏障（BBB）的完整性以及水肿的发展情况。
左侧大脑皮质和尾状核-壳核的血流量减少了70%至80%。31P-MRS和1H-MRS显示氧化磷酸化紊乱。1H-MRI显示血脑屏障即刻破坏，且左半球水肿逐渐进展。
这些结果表明，经高渗造影剂处理的红细胞经颈动脉注入可诱发脑部栓塞及随后的缺血性损伤。
